Queen's Laboratory for Molecular Pathology (QLMP)

QLMP

Queen's Laboratory for Molecular Pathology

site header

Fluorescence In-Situ Hybridization (FISH)

Figure shows a typical schematic of how a typical FISH break-apart probe works

Multiplex fluorescence in situ hybridization (FISH) enables the assay of multiple targets and co-localized signals in a single specimen. Using spectral distinct fluorophore labels for each hybridization probe, this approach gives the power to resolve several genetic elements or gene expression patterns through multicolor visual display.

We offer free initial consultation and set-up.  In all cases the investigator must supply the antibodies and robust positive and negative controls; unless well-validated antibodies are available for the target of interest. 

Like most assays involving nucleic acid binding and detection, the specificity of FISH depends on the nucleotide sequence of the probe and the stringency of the annealing and washing conditions used.  Use the workflow below to determine how your FISH assay shapes up:

Detailed flow chart of the Fluorescent In-situ Hybridization process of customizing > Nucleic acid type > Detection Methods > Target Type